Professor Besik V. Surguladze
SUSPENSIO
MAGNETITE 0,5%
Registration Certificate of pharmacological
committee of Georgia
«0,5% Suspensio Magnetite» is the
suspension of Ultra (High) Dispersive
Magnetite particles.
Active components of «0,5%
Suspensio Magnetite», – Ultra disperse Magnetite particles are spherical form
and 10 – 50 nm. in size. The magnetic particles are not stabilized by surface
active components and aggregates in more big units, 300 – 500 nm. Because of
described aggregation of particles, suspension is un-stabile. Experimental investigations of
medico-biological characteristics show, that "0,5% Suspensio
Magnetite" is characterized by bactericidal and bacteriostatic activity.
Magnetite particles can adsorb and block toxic components as bacterial as
metabolic origin, increase neutrophils phagocytic and mononuclear cells
proliferating activity, stimulate producing of Interleukin – 1 and other
factors of grow by macrophages. «0,5% Suspensio Magnetite» is X-Ray contrast
and magnet-sensitive. «0,5% Suspensio Magnetite» was
registered by pharmacological committee as a remedy for Intraoperative Treating of
Abdominal Cavity During The Operations Because Of Peritonitis. Unimag http://att.host.ge/Unim.htm
Registration
Certificate of pharmacopoeia committee of Georgia
Unimag is the Stabile suspension
(magnetic Fluid) of Ultra (High) Dispersive
Magnetite particles (25 mg. ultra-disperse magnetite in 1 ml of magnetic
fluid). Active components of Unimag , -Ultra-Disperse Magnetite particles are
spherical form and 10 – 50 nm. in size.
Experimental investigations
of medico-biological characteristics show that Unimag is characterized by
bactericidal and bacteriostatic activity. Magnetite particles can adsorb and
block toxic components as bacterial as metabolic origin, increase neutrophils
phagocytic and mononuclear cells proliferating activity, stimulate producing of
Interleukin –1 and other factors of grow by macrophages. Unimag is X-Ray
contrast and magnet-sensitive. At the same time Unimag can easily be loaded by
water solved remedies. Because of high penetrating properties due to
ultra-disperse size of magnetite particles, Unimag easily penetrates in tissue
together with loaded remedies, and furthers successful treating of different
etiology and localized inflamed processes of soft and bone tissue.
METHOD
OF INTRAOPERATIVe treating of abdominal cavity by «0,5% suspensio magnetite»
On the base of the pre-clinical investigations of
general toxicity and the dates of learning the general and specific pharmacological
activity of high disperse magnetite (HDM), at the same time the dates received
by clinical approbation of «0,5% Suspensio Magnetite» was registered by
pharmacological committee of Georgia as the remedy for intra-operation treating
of abdominal cavity during the operation because of peritonitis (clinical
approbation «0,5% Suspensio Magnetite» was cared out in: № 4 clinical hospital
of Tbilisi, Republic sepsis center of Georgia, Central republic hospital of
Georgia, republic Hospital of Kutaisi and et set.).
The analyze of clinical materials showed high
affectivity of the method of intraoperative treating of abdominal cavity by
«0,5% Suspensio Magnetite» in the complex of treating and prophylactics of
pyo-septic complications of peritonitis offered by us.
The Learning of acting mechanisms of «0,5% Suspensio Magnetite» convinced, that
HDM actively takes part in the process of fighting aggressive factors,
compensates some broken functions of detoxification system of organism. The
main question in this direction is the decision of the problem of local
inflamed processes, as the main source of the toxic components. The presence of
the infection agent, destruction of tissue, broken micro circulation and
metabolism increase the possibility of penetration of toxic components of
peritoneal exudates deep in the tissue and bloodstream. The considerable
reduction of the quantity of microbes (Figure I) on the peritoneum after its
intraoperative treating by «0,5% Suspensio Magnetite», is playing main role in
the detoxification of organism.
1- Intraoperative treating of abdominal cavity by 0,5% Suspensio Magnetite”in
the case of peritonitis.
2- Intraoperative treating of abdominal cavity by 1% solution of “Dioxidin” in
the case of peritonitis.
Undoubtedly, decreasing of pathogenic activity of
microbes under the influence of HDM is important, because even after careful
lavage of abdominal cavity by difference solutions, the big quantity of
microbes still stays on the surface of peritoneum, which on the background of
decreasing intensity defensive manifestations of organism, are breading
post-operation period, have ideal thermostatic conditions. Suppression of
growing and toxicity left in abdominal cavity microbes under influence of HDM,
evidently promotes to improve the efficiency of detoxification and
antibacterial therapy. From our point of view, bactericide and bacteriostatic
activity of HDM, apart from destroying the cover of microbes, is the result of
adsorbing and blocking nucleon acids, that do not allows realization of their
pathogenic functions.
Due to high disperse condition, the magnetite
particles easily adsorb the toxic components of peritoneal exudates as
bacterial as well as metabolic origin (Table 1).
The investigations showed, that the toxic components
are blocked due to adsorption on HDM. From our point of view the block of toxic
components is the results of binding their active radicals (I AU of HDM is 1
ml. of suspension in 1/80 rate of solution).
Table № 1
Influence of UM on toxic components of peritoneal exudates
|
Hemolyze indicator |
+ ++ + |
+ + |
- - |
- - |
|
Quantity of UM (AU) |
0,01 |
0,02 |
0,03 |
0,04 |
Morphological investigations showed, that HDM
particles adsorbs on the surface of peritoneum at the places were the
mesotheliocytes are damaged (Figure II), that is, were is bared the basal
membrane, witch will be imaged as open doors for the penetration of aggressive
factors. Seated at this place HDM particles are hindering from the penetration
of infection in the depth of tissue.
Breaking of micro-circulation, catastrophically low
rate of protein in the blood and increasing of metabolic processes leads to the
protein starvation of tissue and breaks the circle of binding of toxins as
bacterial as metabolic origin and their transport thru the membrane of
eliminated cells. Even the macrophages with the high phagocytic activity can
not realizing fully their function with presence of toxins due to lack of
transport units.
HDM particles are recognizing by phagocytes as
antigens and will be absorbed by them (Figure III).
Figure II. High disperse magnetite
particles on the surface of peritoneum. Light microscopy x 300.
If we take into account, that because of high
disperse condition HDM actively adsorbs high molecule radicals including toxic
components, it will be possible to conclude logically that the magnetite
particles will be phagocyted by phagocyte cells together with adsorbents, witch
will be easily eliminated by lysosome ferments. The HDM will be removed from
the cell in not changed condition, because it does not enters in to chemical
reactions with the ferments of lysosomes.
Figure III. Peritoneal macrophages
with HDM particles inside of phagosomes.
Electron Microscopy x20 000
Removed from the cells HDM particles by clasmatose
joins to analogical circle of detoxification as transporter of toxic components
and by this somehow compensate the lesions in the circle of adsorbing and
transport of toxins through the membrane of phagocytes.
Radio-immunologic methods proved that HDM particles
lead to the excretion increasing of Interleukin -1 and other grow factors by
macrophages, which is very important for increasing of reparative processes.
The morphological investigations showed that after
intraoperative treating of abdominal cavity of patients with peritonitis by
“0,5% Suspensio magnetite” the reparation processes in lesion areas begin in
4-5 days early, then in the control (Figure IV).
Figure IV. Reparation of peritoneum
Light microscopy x 300.
It is proved that
adsorbed at the places of damaged mesotheliocytes HDM particles gradually move
in the depth of tissue, penetrate in bloodstream, adsorb on erythrocytes and
will be phagocyted by neutrophils. Phagocyted by macrophages HDM particles move
to lymphatic capillaries (Figure V, VI).
Figure V. Adsorbed on the
erythrocytes and phagocyted by neutrophils HDM particles in the blood
capillaries and macrophages with phagocyted HDM particles in the lymphatic
capillaries. Light
microscopy x 300. Coloring by Hematoxiline-Eosine.
Figure VI. HDM particles in lymphatic nods Light microscopy x 300. Coloring by
Hematoxiline-Eosine
The system of binding and transportation of toxic
components mainly are located in the circulated blood. The buffer systems,
different proteins, form elements of blood and et cet., are related to the
above mentioned.
Accumulation of acid components in the blood in
abundance quantity, break the excretion of and-products and their neutralization
are accompanied with breaking buffer system of organism, decreasing
protein-creating ability of liver and catastrophic hypoproteinosis.
If we take into account the above mentioned,
increasing energetic possibilities and transport function of erythrocytes after
intraoperative treating of abdominal cavity by «0,5% suspensio Magnetite» our
attention will draw. In this direction it is also important to create a native
net by thrombocytes targeting at the binding of toxic components. Learning
morpho-functional alterations of the form elements of blood (Figure VII, VIII)
showed, that because of interaction with HDM particles the lymphocytes acquires
the ability of adsorption and transport of high-molecular radicals of different
origin and by this somehow support detoxification possibilities of organism.
Figure VII. Blood after 12 hour of
intraoperative treating of abdominal cavity
by «0,5% Suspensio Magnetite».
Light microscopy 10 х 2 х 100
Figure VIII. Blood after 48 hour of
intraoperative treating of abdominal cavity
by «0,5% Suspensio Magnetite». Light
microscopy 10 х 2 х 100
The radio-immunologic investigations showed that HDM
is characterized by a comitogen activity, that leads to increasing the
proliferate activity of initiated lymphocytes.
For the stimulation of reactivity of organism the
increasing phagocytic activity of neutrophils (Figure VIIII) is very important.
Investigations showed, that after intraoperative treating of abdominal cavity
of patients with peritonitis by «0,5% Suspensio Magnetite», mainly increases
phagocytic number and phagocytosis completing index. From our point of view the
fact, that neutrophils better absorbs lesion by HDM microbes, plays a big role
in decreasing the seeding rate of peritoneum by microbes in post-operation
period.
The clinical investigations showed, that lowering the
seeding of peritoneum and concentration of toxic components as in pathologic
area as well as in blood stream, increasing of elimination of aggressive
factors, binding of lasts in untoxic complexes, reinforcement transport as
through the membrane of eliminated cells as to the organs of physiological
detoxification due to biological activity of magnetite particles, lead to rapid
regression of inflammation processes and smooth the flow of after-operation
period.
Figure VIIII. Influence of
intraoperative treating of abdominal cavity by “0,5% Suspensio Magnetite”
on phagocytic activity of
neutrophils of peripheral blood of patients with peritonitis in toxic phase.
(1 day after operation)
Profound lesions of homeostasis, due to peritonitis
besides hypoproteinosis leads to the accumulation of a big quantity of
metabolites with breaking nitrogenous balance. Before the operation in control
and in group of observation of patients with disseminated forms of peritonitis
concentration of rest nitrogen in the plasma and urea nitrogen in the serum was
marked high. In all patients the catastrophic hypoproteinosis took place. This
appearance naturally was expressed more in toxic and terminal phases of
peritonitis. In the group of observation in postoperative period faster
normalization of indicators of the rest nitrogen in the plasma comparatively of
control dates took place more (Figure X). We took into account the fact that at
the same time, the concentration of urea nitrogen in serum was not reduced
(Figure XI). From our point of view lowering the concentration of the rest
nitrogen in the plasma, is the result of reducing amino-acid nitrogen due to
active involving of them in the processes of biosyntheses of proteins. Our
opinion on increasing protein-creating function of liver is proved by results
of learning dynamic concentration rate of total protein in the plasma of
patients in the postoperative period (Figure XII).
Figure X. Alteration of concentration
of rest nitrogen
in the plasma of patients operated in the toxic phase of
peritonitis.
Figure
XI Alteration of concentration of urea nitrogen
in
the serum of patients operated in
the toxic phase of peritonitis.
Figure
XII Alteration of concentration of total protein
in
the plasma of patients operated in
the toxic phase of peritonitis.
The investigations showed, that before operation in the patients of both
groups the concentration of urea nitrogen in the serum was moderately high and
stood at the top of the norm. In the postoperative period in the patients
control group together with, it is true, insignificant, but nevertheless
improvement of excretory function of kidneys (Figure XIII), sharp lowering of
concentration of urea nitrogen in serum took place. The above mentioned
indicates that the before operation in the serum of patients with peritonitis
increasing concentration of the last was not the result of increasing of
syntheses of urea in the liver, but it was caused by lesion of excretory
function of kidneys due to increasing endogenic intoxication of organism. As
for patients of the observation group, in this case, in the postoperative
period sharp dynamic of increasing of concentration of urine nitrogen in the
urine took place. However in spite of the considerable improvement of excretory
function of kidneys, the concentration of urea nitrogen in the serum was not
lowering and had certain tendencies of increasing. This fact indicates the
considerable increase of urea-genesis function of liver patients with
disseminated forms of peritonitis. The investigations showed, that HDM is
comitogens. We took into account and mentioned the fact that in case of
peritonitis, the lymphocytes, from the very beginning meets aggressive factors,
we can easily explain faster stabilization of immune system of patients of the
observation group comparatively with the control one.
Figure
XIII. Alteration of concentration of urea nitrogen
in
the urine of patients operated in
the toxic phase of peritonitis.
Besides the improvement of functional condition of liver, we drew our
attention to the considerable increasing of reactivity of T-cells unit of
immunity after intraoperative treating of abdominal cavity of The received
results prove the lesion of immune status with all phases of peritonitis, that
is expressed by lowering total quantity of T lymphocytes (T3), helper-inductors
(T4) and raising the quantity of suppressor-cytotoxic (T8) cells with sharp
lowering of correlation index of regulating cells T4/T8 (Figures XIV, XV, XVI).
Figure
XIV Alteration of quantity of T3 cells
in
the peripheral blood of patients operated in the toxic phase of peritonitis
Figure
XV. Alteration of quantity of T4 cells
in
the peripheral blood of patients operated
in the toxic phase of peritonitis
Figure
XVI. Alteration of quantity of T8 cells
in
the peripheral blood of patients operated in the toxic phase of peritonitis
The level of lesion of T cells unit of immunity, in particular, lowering
the quantity of lymphocytes with phenotypes HP3+ and HP4+ directly relates with
the depth of illness.
The intraoperative treating of abdominal cavity of patients with
peritonitis by “0,5% Suspensio Magnetite” leads to the faster normalization of
total quantity of lymphocytes, a sharp raising of quantity of helper-inductors
and accordingly lowering quantity of suppressor-cytotoxic cells. In the results
mentioned, the raising of correlation index of regulating cells took please.
Alike picture is observed in all phases of disseminated peritonitis. From our
point of view, the sharp inverse of regulating cells, that is, the decline of
correlation index out of norm is the compensating appearance.
As it was mentioned above, rising of quantity of adult lymphocytes in
the periphery blood of patients is more intensive in the group of observation.
Probably under the influence of “0,5% Suspensio Magnetite” differentiation of
adult lymphocytes mainly toward the helper-inductors takes place, that promotes
the restoration of lesion balance of given cells. From our point of view the
lowering quantity of lymphocytes with phenotypes HP8+ in the periphery blood of
patients under the influence of “0,5% Suspensio Magnetite” is the result of
mobilization of cytotoxic cells in the zone of lesion.
Lowering seeding of peritoneum and toxicity of microbes left into
abdominal cavity after its intraoperative treating by “0,5% Suspensio
Magnetite”, binding and blocking toxic components of peritoneal exudates,
increasing of mechanisms of adsorption and transportation of toxins, functional
condition of liver, reactivity of T cell unit of immunity and phagocyte
activity of eliminating cells lead to the sharp lowering of endogenic
intoxication of organism.
Figure
XVII. Dynamic of test of Paramecia
in
patients operated in the terminal phase of peritonitis.
The investigations showed that in the group of observation faster
normalization of indicators of plasma toxicity (test of Paramecia and leukocyte
index of intoxication) then in control (Figure 17, 18) took place.
Figure XVIII. Dynamic of leukocyte index of intoxication (LII)
in patients operated in the
terminal phase of peritonitis.
Increasing of proliferate processes in the zone of lesion and lowering
endogenic intoxication by their side promotes the improvement of treating
effects.
The analysys of clinical material showed the efficiency of the
intraoperative method treating abdominal cavity by “0,5% Suspensio Magnetite”.
In the group of observation more less percent of postoperative
complications are revealed comparatively with the control one (Figure XVIIII).
Figure XVIIII. Postoperative
complications in patients with disseminated forms of peritonitis.
The
duration of stationary treating of patients was statistically reliable
(P<0,05) lowering in the group of observation, comparatively with the
control one. It must be mentioned that the effect was more expressed in the
toxic and terminal phases of the disease.
The
lethality of patients, as in toxic as in terminal phases of peritonitis, was
statistically reliable (P<0,05) lower in the group of observation
comparatively with the control one (Figure XX, XXI).
It must be mentioned, that the good results ware received not only in
the case of the use of intraoperative treating of abdominal cavity by “0,5% Suspensio
Magnetite”in the patients with peritonitis but also for the prophylactic of
pyo-septic complications in the case of non penetrating traumas and penetrate
wounds of abdomen cavity.
The results of clinical investigations allow to conclude that therapeutic
effect of «0,5% Suspensio Magnetite» is concerning not only the mechanical
cleaning of peritoneum, but lot of medico-biological characteristics of HDM
witch promotes the increasing of defensive mechanisms of organism.
Figure XX. Average duration of stationary treating of patients.
Figure XXI. Lethality in patients with disseminated forms of
peritonitis: 1.Toxic phase; 2.Terminal
phase.
MEDICO – BIOLOGICAL
CHARACTERISTICS OF MAGNETIC
FLUIDS
Offered by us (Scientific group of Professor Besik V. Surguladze)
Magnetic Fluids represents suspension of ultra disperse magnetite particles on
water bases.
Ultra (high) disperse Magnetite (in future UM) Fe3O4
is received by the method of chemical condensation of (II) and (III) Iron salts
in abundance of NH4OH (Method was elaborated by W. Elmore in 1938,
and improved by N.Gribanov in 1986).
Ultra disperse Magnetite particles, received by the method described
above, are 10 – 50 nm in size, have spherical form. They are X-Ray contrast and
magnet-sensitive.
Experimental investigations of medico-biological characteristics show
that UM is characterized by high bactericidal and bacteriostatic activity.
After adsorption on the surface and aggregation, UM destroys the cover of
microbe and leads it to death (Figure XXII, XXIII, XXIV, XXV, XXVI).
Figure XXII. Staphylococcus with
magnetite particles on the surface. Electron microscopy X 120.000
Figure XXIII. Aggregation of
magnetite particles around the staphylococcus.
Electron
microscopy X 60.000.
Figure XXIV. Staphylococcus with destroyed cover due to aggregation of
magnetite particles.
Electron
microscopy X 120.000.
Figure XXV. Escherichia coli with magnetite particles on the surface.
Electron
microscopy X 60.000.
Fugure XXVI. Escherichia coli with destroyed cover due to aggregation of
magnetite particles.
Electron microscopy X 60.000.
The investigations show that for destroying one microbe 107
ultra disperse particles are necessary. In the case of adsorption of ultra
disperse particles in quantity less then 107, microbes do not die
but decrease their reproductive, toxin production and hemolyze activity.
The results of the biological test show that UM adsorbs and blocks
toxins. Adding of UM in different rate of solution on standard thermo-stabile
extra cellular toxin of staphylococcus shows, that ultra disperse magnetite
only in the rate 1/100 of solution can not block 1 Lh of extra cellular toxin
(Table № 1). In the case of using UM in the rate of 1/80 solution the result of
the test was semi-positive. It means that 1 ml of UM suspension in 1/80 rate of
solution (1017-1018 ultra disperse particles) is 1 AU.
Table № 1
Biological test for the determine 1AU of UM.
|
Hemolyze
indicator |
- - |
- - |
- - |
+ + |
+ ++ + |
+ ++ + |
|
Solution rate of UM |
1/20 |
1/40 |
1/60 |
1/80 |
1/100 |
1/200 |
The investigations directed to the learning of interaction of UM with
toxic components of peritoneal exudates show that magnetite particles can
adsorb and block toxic components as bacterial as metabolic origin.
Semi-positive blocking of 0,5 ml of LD100 peritoneal exudates occurs
in case of adding of 0,02 AU of UM (Table № 2). From our point of view blocking
of toxic components is the result of binding of functional unites of toxic
molecules during of adsorption on the ultra disperse particles.
Table № 2
Influence of UM on toxic components of peritoneal exudates.
|
Hemolyze
indicator |
+ ++ + |
+ + |
- - |
- - |
|
Quantity of UM (AU) |
0,01 |
0,02 |
0,03 |
0,04 |
Learning of influence of UM on functional activity of mononuclear cells
show that ultra disperse magnetite increases proliferating activity of
mononuclear cells preliminary stimulated by lectin. The results, received by
adding of UM on the mononuclear cells after their stimulation by lectin are
statistically reliably (P<0,05) high comparatively with the results received
by adding of only lectin (Figure XXVII). It is necessary to note, that the
adding of only magnetite do not lead to the increasing of proliferating
activity of mononuclear cells (P>0,05). The described dates indicate that UM
is characterized by co-mitogenic activity.
The reason of the the definition, was the received effect consequence of
direct action of UM on lymphocytes or it was based on increasing of functional
activity of macrophages due to interaction with the magnetite particles, we
conducted the investigations by dividing mononuclear cells on adhesive and not
adhesive parts. Adding only lectin or lectin together with UM to separated
lymphocytes do not lead to the increasing of proliferating activity of cells
(P>0,05), while the adding of supernatant of macrophages preliminary
stimulated by lectin or lectin together with UM, lead to the significant
increasing of proliferating stimulation index of lymphocytes (Figure XXVIII).
Figure XXVII. Influence of UM on proliferate activity of mononuclear
cells.
Figure XXVIII. Investigations by
dividing mononuclear cells on adhesive and not adhesive parts.
The results received by adding of supernatant macrophages preliminary
stimulated by lectin together with UM on separated lymphocytes are
statistically reliably (P<0,05) high comparatively with the results received
by stimulation of macrophages only by lectin (Figure XXVIIII). The received
results provide the possibility to conclude, that magnetite particles increase
the producing of Interleukin -1 and other factors of grow by macrophages.
The investigations directed to the learning of influence of UM on
neutrophils phagocytic activity show that the magnetite particles are
statistically reliable(P<0,05) increasing phagocytic number and phagocytosis
completing index, comparatively with the control dates. It was also observed
that the tendencies increasing of phagocytic index bat the results were not
statistically reliably (P>0,05) different comparatively with control dates
(Figure XXVIIII).
Figure XXVIIII. Influence of UM on phagocytic activity of neutrophils.
Phagocytic number and phagocytosis completing index was especially
increased when microbes preliminary treated by UM was added on the mass of
leukocytes . The described results was statistically reliably high
comparatively with control dates as well as the results received by adding
intact culture of staphylococcus on the neutrophils preliminary treated by UM
(P<0,05, P1<0,05).
UM is recognizable by phagocytes as antigens and are exposed by phagocytosis,
but it can not be eliminated by lysosome ferments and magnetite particles leave
the cell by clasmatosis (Figures XXX, XXXI).
Figure XXX. Schematic presentation of processes of phagocytosis and
clasmatosis.
Figure XXXI. Neutrophile with phagocited magnetite particles and processes
of clasmatosis.
Light microscopy, coloring by method of Andres, 10X2X100
Pre clinical examination of UM show that magnetite particles are not
only characterized by the local-irritation and allergic actions, but also with
mutagen activity. UM is so un-toxic that it is impossible to determine even the
LD50.
On the bases of the research results described above, for the first time
in the world we registered in the pharmacological committee two kind of drugs
for treating peritonitis and other pyo-septic processes: -"0,5% suspension of magnetite" and -"Unimag".
Some aspects
of bio – energetic transformation in alive
system
Today it is considered, that the oxidation reactions in the cells are
the sours of supplies all the vital processes by energy.
It is impossible to calculate general energy –
U of alive system. But if system receives a certain quantity of energy
assume as heat – Q, after some time it will be
presented as energy spent on execution of useful work –
∆W, energy lost on reinforcement of entropy –T∆S (where, T is
absolute temperature) and remained free energy –
∆G accessible for the execution of useful work.
Q = ∆W + T∆S + ∆G (1)
The executed useful work in alive system will be imagined as energy spend
to keep organism in high-organized, otherwise far from balance condition.
According to today’s imagination it’s a part of energy spent on creating ATP
and other high-molecular matters necessary for the homeostasis, ion dis-balance
with cells and area between of cells, etc. By other words, the execution of
useful work can be imagined as transformation of free energy in potential
chemical energy Epch which after some time can be used again by the
alive system for receiving free energy:
∆W 
∆G =
∆Epch (2)
Taken into
account the mentioned can be written
Q = (∆Epch ∆G) + T∆S (3)
The mass and the composition of alive system remaining invariable in
spite of a lot of oxygenation reactions and the permanently changed environment
that is based on the existence of subtle mechanisms of operation of energetic
processes.
It’s a wonder, how it happens, that for the transformation of received
energy (as heat from outside or due to oxygenation reactions in chemical
energy) by the way of creating ATP or other high-molecule maters, the exactly
certain quantity of certain elements in certain space, sharply in certain time
of released energy presence in critical distance necessary for the execution of
chemical reaction. It mast be received in account that energy released during
disintegration of one glucose molecule it is enough for creating 38 ATP
molecules.
If we do not admit existence of other labile buffer unite of energy
transformation and leaning toward the opinion, that the part of the released
energy which was not used for the execution of useful work will be lost only as
heat, it is not clear how alive system is successful to keep the temperature in
the borders of norm. If we take into account that surface of alive system has
possibility of heat-exchange with the surrounding, but the heat released into
deep strums of tissue passes to the nearest structures which by their side also
produces heat (it’s known that the relation of the heat production with the
mass of alive system change widely for the different species while its relation
with the area of surface always is permanent) we will see that it could be
ineffective and fatal for the organism.
A lot of examples of transformation energy exist in the nature, received
by alive system from of oxygenation reactions or by any other way, not only in
chemical energy and heat but also in another forms of energy as electrical,
light etc.
During the estimation of metabolic processes passed in alive system the
possibility of absorbing or receiving energy in corpuscular or waive forms is
ignored, notwithstanding absorbing of electro-magnetic waves based on good
known method of electro-paramagnetic-resonance spectroscopy as a better way of
analyses free radicals maintenance; Photon absorb principle is used in
spectral-photometric investigations; It mast be taken into account influence of
ionization energy on alive system.
Stability and chemical activity of atoms causes by periphery electrons.
In the intact condition of surface atom is stabile, but in the case of presence
more or leas electrons expresses tendency of restoration of atom balance. Alive
system, alike of another mater absorbs passed corpuscular or waive energy,
destroys energetic balance of atom and leads to its chemical activity. The
namely alike appearances takes place during the influence of radiation
(ionization) on alive system. Especially important ionization and excitement.
Atom excitement Energy will be passed to another atoms inside of molecule, it`s
named inner converse. The transmission
of energy is possible from excitement atom to the atoms of another nearest
molecule. This is named passage or not-radiation converse. Excitement, but not
dissociated molecule after some time loses and releases excitement energy and
return to the beginning condition, conducting a like as atoms under the thermal
influence.
Transformation of released due to electrons moving inside of energetic
orbit energy in heat or another kind of energy influences on execution of
chemical reactions. Energetically characteristics of free radicals non-stabile
electrons determine the oxygenation-restoration potential of alive system. The
difference between electric potentials is the expression of the free energy,
especially important in thermodynamic notion.
The lack of the free energy as abundance of it, leads to the development
of disease. Due to bio-energetic processes, permanent transformation of free
energy takes place that by its side naturally leads to the abundance or the
catastrophic deficit of energy in any conditional volume unit of tissue.
Without existence of subtle mechanisms of transmission and transformation of
energy, it could be imaginable keeping of homeostasis and control of
permanently passing vital processes.
From our point of view excitement of atoms of alive system, inner and
not-radiation converses have please not only under the ionization radiation,
but in the case of receiving of any kind of energy, as released during
oxygenation reactions as from outside of system in any form (Figure XXXII).
Figure XXXII. Model of Bio-Energy transformation in alive system.
Moving of electron inside of atom on highest level inside of own
energetic orbit must give fast, labile possibility of cumulation and
transmission of energy, what practically will be imagined as buffer energetic
unite – Eb.
∆W ∆G = ∆Epch + ∆Eb (4)
Energy transformation buffer must be discussed as middle unit in today
admitted popular scheme of cumulation and usage of energy for the execution of
useful work. Took into account above described № 3 equation will be expressed
as shown below
Q = [(∆Epch+ ∆Eb) ∆G] +
T∆S (5)
Expressed equation is right in any cases receiving of energy by alive
system in any form.
From our point of view, the oxygenation-restoration potential and free
radicals quantity oscillation in limited frames of norm in alive system bases
on stability of comparative maintenance of water (its dissociated elements) in
volume unit of tissue. Exactly of water molecules free radicals valence
electrons energy-capacity inside of
energetic orbit and their possibilities of receive and pass energy in
corpuscular or waive forms presents basis of existence of bio-energetic
transformations labile buffer unite in alive system.
The offered by us model of energy-transformation in alive system do not
deny and nor contradict the principles admitted today, on the contrary filings
them and gives possibility of better comprehend the passing in alive system
energetic processes.
PUBLICATIONS:
1. Ахалая М. Г., Табагуа М. И.,
Вольтер Е. Р., Какиашивли М. С. Изучение микроструктуры и магнетизма на
поверхности модифицированных малых частиц магнетита. Тбилиси: ГрузНИИНТИ, № 120Г
– 54 Депонент 14 с.
2. Ахалая М. Г., Какиашвили М.
С., Берия В. П., Вольтер Е. Р., Табагуа М. И. Магнитные жидкости – управляемые
регуляторы биоэнергетических процессов. Труды IV Всесоюзной
межуниверситетской конференции по биологии клетки. Тбилиси: ТГУ, 1985.
3. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р. Электронные поверхностные состояния в частицах феррофазы
магнитных жидкостей. Тезисы докладов IV Всесоюзной конференции по
магнитным жидкостям. Иваново: ИЭИ, 1985.
4. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р. Магнитные жидкости и регуляция биоэнергетических процессов.
Тезисы докладов II конференции по применению магнитных жидкостей в
биологии и медицине. Сухуми: ГИСХ, 1985.
5. Ахалая М. Г., Вольтер Е. Р.,
Какиашвили М. С., Санеблидзе Л. Г., Хабурзания Г. П. Автоколебательные
электрохимические процессы в магнитных жидкостях. Тезисы докладов III
Всесоюзного совещания по физике магнитных жидкостей. Ставрополь, 1986.
6. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р. Влияние защитных оболочек и кислорода на микроструктуру
дисперсных частиц магнитных жидкостей. В книге: структурные свойства и гидродинамика
магнитных коллоидов. Свердловск: УНЦ АН СССР, 1986, С. 35 – 43.
7. Ахалая М. Г., Какиашвили М.
С., Закарая К. А., Вольтер Е. Р. и другие. Исследование адсорбционных свойств
магнитных жидкостей медицинского назначения. Тезисы докладов XII
Рижского совещания по магнитной гидродинамике. Саласпилс: ИФ АН
Латвийской ССР, 1987, том 4.
8. Ахалая М. Г., Алёхин В. П.,
Вольтер Е. Р., Гальченко Ю. Л. Исследование ферромагнитного резонанса в
коллоидах магнетита. М.: Депонент ВИНИТИ № 8692-B 88. 8c.
9. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р. Изучение свойств магнитных жидкостей методом
ЭПР-спектроскопии. Тезисы докладов V Всесоюзной конференции по
магнитным жидкостям. М.: МГУ, 1988.
10. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р., Табагуа М. И. К вопросу о струткуре магнитной жидкости.
Тезисы докладов IV Всесоюзного совещания по физике магнитных
жидкостей. Душанбе: ТГУ, 1988.
11. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р. Экспериментальное исследование и моделирование динамики
магнитных жидкостей в организме. В кн.: Биокибернетика и биофизика.
Тбилиси. Мецниереба, 1989, С. 8 – 28.
12. Ахалая М. Г., Бибик Е. Е.,
Вольтер Е. Р., Грибанов Н. М.,
Какиашвили М. С., Наумов В. Н. Принципы моделирования транспорта магнитной
жидкости в организме. Тезисы докладов III Всесоюзной конференции по
применению магнитных жидкостей в биологии и медицине. Сухуми, 1989.
13. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р., Нечепуренко Э. А., Чавчанидзе М. Г. Получение и
использование магнитных жидкостей с легированной феррофазой. Тезисы докладов XIII
Рижского совещания по магнитной гидродинамике. Саласпилс: ИФ АН Латвийской ССР,
1990.
14. Ахалая М. Г., Какиашвили М.
С., Вольтер Е. Р. Фрактальные агрегаты в ферроколлоидах. Тезисы докладов V
Всесоюзного совещания по физике магнитных жидкостей. Пермь, 1990.
15. Ахалая М. Г., Бибик Е. Е.,
Вольтер Е. Р., Грибанов Н. М.,
Какиашвили М. С., Наумов В. Н.
Капиллярные и термофлуктуационные эффекты при адагуляции коллоидного магнетита
на бактериальной стенке. Тезисы докладов IV Всесозной конференции по
применению магнитных жидкостей в биологии и медицине. Сухуми, 1991.
16. Ahalaya M. G., Kakiashvili M. S., Volter E. R. Investigation of the
mechanism of magnetic fluid interactions with biological structures. Fifth
International conference on magnetic fluids. Abstracts.
17. Ahalaya M. G., Kakiashvili M. S., Volter E. R. Antiinflammatory effects
on magnetic fluid. Sixth International conference on magnetic fluids.
Abstracts.
18. Kakiashvili M. S., Volter E. R. Influence of protective shells and
oxygen on the microstructure of magnetic particles in magnetic fluids.
Fluidmex, 1989, V. 18, № 6, P. 72 – 77.
19. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Закарая К. А., Какиашвили М. С., Табагуа М. И. Способ получения магнитной
жидкости для биологических исследований. А. С. № 1185804, Гриф «Т».
20. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Какиашвили М. С., Табагуа М. И. Способ определения количества
жизнеспособных клеток бактерий. А. С. № 1400283, ДСП.
21. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Какиашвили М. С., Табагуа М. И. Способ получения магнитной
жидкости. А. С. № 1403795, ДСП.
22. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Какиашвили М. С., Табагуа М. И. Способ определения уровня
активности фагоцитоза лейкоцитов. А. С. № 1403796, ДСП.
23. Ахалая М. Г., Вольтер Е. Р.,
Какиашвили М. С., Табагуа М. И. Способ получения коллоидного раствора магнетита.
А. С. № 1405600, ДСП.
24. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Какиашвили М. С., Табагуа М. И. Способ моделирования токсической
гепатопатии. А. С. № 1628075, Б. И.,
1991, № 6.
25. Ахалая М. Г., Вольтер Е. Р.,
Какиашвили М. С., Сургуладзе Б. Г., Эмухвари Д. Г. Способ моделирования
асептического перитонита. А. С. № 1649595, Б. И., 1991, №
18.
26. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Какиашвили М. С., Какубава В. В. Сопосб моделирования
иммунодефицитного состояния. А. С. № 1681669,
ДСП.
27. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Какиашвили М. С. Способ моделирования гиперкоагулемии. А. С. №
1707618, Б. И., 1992, № 3.
28. Ахалая М. Г., Вольтер Е. Р.,
Какиашвили М. С., Какубава В. В., Табагуа М. И. Способ моделирования
нефропатии. Заявка № 4902948/14 (005968). Положительное
решени от 21. 06. 91.
29. Ахалая М. Г., Вольтер Е. Р.,
Какиашвили М. С., Хачатрян Р. М. Способ индикации анаэробных бактерий в
биологическом материале. А. С. № 1757323,
ДСП.
30. Ахалая М. Г., Вольтер Е. Р.,
Дзидзигури Д. Ш., Какиашвили М. С., Какиашвили Я. М. Способ получения коллоидного раствора
магнетита. А. С. № 1817443, ДСП.
31. Ахалая М. Г., Берия В. П.,
Вольтер Е. Р., Какиашвили М. С., Санеблидзе Л. Г. Способ моделирования эпителиоидной гранулемы.
Заявка № 4939672/14 (044208). Положительное решение от 03. 01. 92.
32. Вольтер Е. Р., Глущенко Н.
Н. Физико-химические аспекты применения магнитных жидкостей в экспериментальной
биомедицине. // Сборник научных трудов IX Международной Плёсской
конференции по магнитным жидкостям. Иваново: ИГЭИ. 2000. С. 349 – 351.
33. Вольтер Е. Р., Какубава В.
В., Кикория А. Д., Будник М. Н., Губин В. В. Применение коллоидного магнетита
для модуляции биологического действия электромагнирного излучения
КВЧ-диапазона. Материалы Российской конференции «Организм и окружающая среда:
жизнеобеспечение и защита человека в экстремальных условиях». Москва, Слово, 2000, Том 1, C. 98 – 99.
34.
Kakubava V. V., Kikoria A.
D., Volter E. R. In heterointeraction of purnient round microbes and
35.
colloidal magnetite
particles, International symposium of magnetic carriers. Biological and
Clinical Applications (ISMC – 1999). Book of abstracts. Wubian.
36. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Results of microbiological
investigations after treating of abdominal cavity by Magnetic Suspension during
the operations because of peritonitis // «Questions
of Surgery of abdominal cavity», Tbilisi, 1989.
37. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Treating of abdominal cavity by
Magnetic Suspension during the operations because of peritonitis in experiment.
// «Vestnik» of Surgery № 4,
38. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Magnetic Suspension for Treating of peritonitis in
experiment. // III Conference “Magnetic fluids in Medicine and Biology”,
Collections of articles,
39. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Influence of ultra-dispersive magnetite on microbes // III
Conference “Magnetic fluids in Medicine and Biology”, Collections of articles,
Sukhumi, 1989.
40. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Colloidal magnetite for Treating of peritonitis in
experiment. // Methodic Recommendations,
41. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Lavage of intestine by Magnetic Suspension in the case of
ileac passion in experiment. // XII Congress of surgeons, Collections of
articles,
42. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Pharmacokinetics of ultra-dispersive magnetite after
treating of abdominal cavity by Magnetic Suspension during the operations
because of peritonitis. // XX Conference of medical scientists of
43. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Leukocyte index of intoxication after treating of abdominal
cavity by Magnetic Suspension during the operations because of peritonitis. //
Scientific conference honored to 85 anniversary of Academician M.Komakhidze,
Collections of articles, 1991.
44. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Standardization of Magnetite Suspension. // Scientific
conference “New methods and remedies for treating of abdominal cavity in the
case of peritonitis”,
45. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Mechanisms of Antitoxic and Immuno-stimulate activity of
magnetite. // Scientific conference “New
methods and remedies for treating of abdominal cavity in the case of
peritonitis”,
46. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Intra-operating treating of abdominal cavity by Magnetic
Suspension in the case of peritonitis. // Scientific conference “New methods
and remedies for treating of abdominal cavity in the case of peritonitis”,
47. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Using of Magnetic Suspension in the cases of traumatic
injury of organs of abdominal cavity for the prophylactic and treating of
septic complications. // Scientific conference “New methods and remedies for treating of
abdominal cavity in the case of peritonitis”,
48. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Morpho-functional alterations of blood cells after treating
of abdominal cavity by Magnetic Suspension during the operations because of
peritonitis. // Annuals of of science
49. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Medico-Biological
characteristics of
ultra-dispersive magnetite particles. //
“Science”,
50. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Method of treating of
abdominal cavity by Magnetic Suspension
during the operations because of peritonitis. // Methodic
Recommendations,
51. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Standardization of magnetite
suspension. // Methodic Recommendations,
52. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Influence of
Intra-operating
treating of abdominal cavity by Magnetic
Suspension in the case of peritonitis on immune status of
patients. // Dep. in scientific
53. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Influence of ultra-dispersive
magnetite particles on Phagocytic
activity of periphery blood neutrophils. // Dep. in scientific institute
of Sc.Techn. Information of
54. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Antitoxic characteristics of
ultra-dispersive magnetite particles.
// Dep. in scientific
55. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Influence of Intra-operating
treating of abdominal cavity by Magnetic
Suspension in the case of peritonitis on dynamic of
endogenic intoxication of organism.
// Dep. in scientific
56. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Some medico-biological
characteristics of ultra-dispersive
magnetite. // Monograph,
57. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Blast-transformation of
lymphocytes after influence of
cytostatics, immobilized on ultra-dispersive magnetite particles
covered by poliacrilamide. // Dep. in
scientific
№ 867, 1993.
58. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Investigations of cytostatic
activity of immobilized chemotherapeutic
remedies on the culture of breast cancer cells. // Dep. In
scientific
59. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Culture of ovary cancer cells
as object of Investigations of anticancer
activity of immobilized chemotherapeutic remedies. // Dep.
in scientific
60. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Investigations of cytostatic
activity of immobilized chemotherapeutic
remedies on the culture of ovary cancer cells. // Dep. In
scientific
61. Tskitishvili T., Surgyladze B., Chelidze
L., Bagishvili A., Shanidze M. «Carcino-sarcoma Uocer-256»
as
object of Investigations of anticancer activity of immobilized chemotherapeutic
remedies in
experiment. // Dep. in scientific
62. Tskitishvili T., Surgyladze B., Chelidze
L., Bagishvili A., Shanidze M. Negative influence of
Targeting chemotherapy. // Collections of
scientific articles,
63. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Using of ultra-dispersive
magnetite particles for the targeting thermo-chemotherapy of cancers. // Georgian Medical
Annuals,
64. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Physico-chemical
characteristics of magneto-sensitive containers and
container-citostatics. // Dep. in scientific institute
of
Sc.Techn. Information of
65. Tskitishvili T., Surgyladze B., Chelidze
L., Bagishvili A., Shanidze M. Pharmacokinetics of
Doxorubicine immobilized on magneto-sensitive containers. // Dep. in scientific institute of
Sc.Techn. Information of
66. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Influence of magneto-
sensitive containers on morphology of
blood cells. // Dep. in scientific
Information of
67. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Influence of magneto-
sensitive
containers on Phagocytic activity of periphery blood neutrophils. // Dep. in
scientific
68. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Influence of magneto-
sensitive
containers on proliferate activity of mononuclear cells. // Dep. In scientific institute of
Sc.Techn.
Information of
69. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Magnetically guided drugs
based on magnetic poliacrilamide
particles. // International conference «soil of surroundings»
Collection of articles,
70. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Cytostatic action of
magnetically guided drugs based on magnetic poliacrilamide particles. //
International symposium
«materials
and technologies», Collection of thesis’s,
71. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Structural changes of blood
corpuscles after intravenous injection of
magnetic poliacrilamide particles. // Int. Fed. of Clinical
chemistry XV World Congress.
72. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Cytostatic action of magnetic
poliacrilamide particles. // Int. Soc. Of Chemotherapy. XVIII
73. Tskitishvili T.,
Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Influence of
Intra-operating
treating of abdominal cavity by Magnetic
Suspension in the case of peritonitis on functional activity
of liver. // Georgian Medical Annuals,
74. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Influence of Intra-operating
treating of abdominal cavity by Magnetic
Suspension in the case of peritonitis on detoxification
activity of liver. // Conference «Actual
questions of military medicine», Collection of articles,
75. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. A new type of targeted
chemotherapy of malignancies. // Turkish
journal of oncology.
76. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Magneto sensitive
Thermo-Chemotherapy of breast cancer. // «Mamology»,
77. Tskitishvili T.,
Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Medico-Biological
aspects
of Intra-operating treating of abdominal
cavity by Magnetic Suspension in the case of peritonitis.
// Symposium “medico-Biological problems
of Surface chemistry”,
78. Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Morpho-functional alterations
of bone marrow after intra
peritoneal infusion of magnetic fluid in radiation-damaged animals.
// Dep. in scientific
79.
Tskitishvili T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Effects
of intra peritoneal
infusion of magnetite suspension on the
development of postoperative adhesions. // Dep. in scientific
80. Tskitishvili T., Surgyladze B., Chelidze
L., Bagishvili A., Shanidze M. Alteration of homeostasis in
the
case of Intra-operating treating of abdominal cavity by Magnetic Suspension in
the case of
peritonitis. // Dep. in scientific
81. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Results using of suspension
magnetite for Intra-operating treating of
abdominal cavity by Magnetic Suspension in the case of
peritonitis. // Dep. in scientific
82. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. For the question of Intra-
operating treating of abdominal cavity by
colloid solution of Magnetite in the case of peritonitis.
// Dep. in scientific
83. Tskitishvili T., Surgyladze B., Chelidze L.,
Bagishvili A., Shanidze M. Energetic buffer in the cycle
of energy
transformation in alive system. // III international congress «Subtle Fields in
Biology and
Medicine»
Sankt-Peterburg, 2003.
84. Tskitishvili
T., Surgyladze B., Chelidze L., Bagishvili A., Shanidze M. Medico- biological
characteristics of preparation UNIMAG. //
Georgian Medical News, New-York,
2004, № 2 (107) .
85. Tskitishvili T., Surgyladze B.,
Chelidze L., Bagishvili A., Shanidze M. Medico-biological
characteristics of Magnetic Fluids. // 5-th Intern. Сonf. "Sci. and Clin. Appl. of Magnetic
Cariers,