Ivo Safarik
Associated Professor,
Doctor of philosophy ( PhD. ),
Doctor of science ( DSc ).
E – mail: safarik@uek.cas.cz
ivosaf@yahoo.com
BIOMAGNETIC
RESEARCH AND TECHNOLOGY
Welcome on my
personal WWW pages, which are mainly focused on the biomagnetic
research and technology. I intend to bring here important information from
this interesting area.
· My papers focused on biomagnetic research and technology
· My papers focused on applied enzymology and biochemical analysis
· Biomagnetic research and technology (explanation of terms)
· BioMagnetic Research and Technology (a new on-line journal)
List
of papers focused on biomagnetic research and technology
(only journal
papers and book chapters are presented)
1.
Safarik,I.: Magnetic biospecific affinity adsorbents
for lysozyme isolation. Biotechnol. Techniques 5 (1991) 111-114.
3.
Safarik,I., Safarikova,M.: Magnetic separation of
microbial cells. Chem. Listy 88 (1994) 464-471.
6.
Safarik,I.,
Safarikova,M.: Construction of a simple flat magnetic separator. Biotechnol.
Techniques 9 (1995) 137-138.
10. Safarikova,M., Nymburska,K.,
Blazek,Z., Safarik,I.: Rapid removal of magnetic particles from large volumes
of suspensions. Biotechnol. Techniques 10 (1996) 391-394.
11.
Safarikova,M., Safarik,I.: The use of copper
phthalocyanine dye immobilized on magnetic carriers for the isolation of planar
organic compounds. Ecol. Chem. 5 (1996) 205-209.
13. Safarik,I., Safarikova,M.:
Overview of magnetic separations used for biochemical and biotechnological
applications. In: Scientific and Clinical Applications of Magnetic Carriers
(Hafeli,U., Schutt,W., Teller,J., Zborowski,M., Eds.). Plenum, New York, 1997,
pp. 323-340.
17.
Safarikova,M., Safarik,I.:
Magnetic solid-phase extraction. J. Magn. Magn. Mater. 194 (1999) 108-112
22.
Safarik,I.,
Safarikova,M.: Cells: Magnetic techniques. In: Encyclopedia of Separation
Science (Wilson,I.D., Adlard,T.R., Poole,C.F., Cool,M., Eds.), Academic Press
Ltd., London, 2000, pp. 2260-2267
30.
Safarik,I.,
Safarikova,M.: Magnetic nanoparticles and biosciences. Monats. Chem. 133 (2002)
737-759
31.
Safarik,I.,
Ptackova,L., Safarikova,M.: Adsorption of dyes on magnetically labeled baker's
yeast cells. Eur. Cells Mater. 3 (Suppl. 2) (2002) 52-55
32.
Safarikova,M.,
Safarik,I.: Interaction of tricyclic drugs with copper phthalocyanine dye
immobilized on magnetic carriers. Eur. Cells Mater. 3 (Suppl. 2) (2002) 188-191
33.
Safarikova,M., Safarik,I.: Magnetic solid-phase extraction of target
analytes from large volumes of urine. Eur. Cells Mater. 3 (Suppl. 2) (2002)
192-195
34.
Safarik,
I., Safarikova, M.: Magnetic nanoparticles and biosciences. In: Nanostructured
materials (H. Hofmann, Z. Rahman, U. Schubert, Eds.). Springer-Verlag, Wien,
2002, pp. 1-23.
37. Safarikova,M.,
Roy,I., Gupta,M.N., Safarik,I.: Magnetic alginate microparticles for
purification of α-amylases. J.
Biotechnol. 105 (2003) 255-260
39. Safarik,I., Safarikova, M.:
Magnetic nano- and microparticles in biosciences and biotechnology. In:
Nanoscale magnetic oxides and bio-world (I. Nedkov and P. Tailhades, Eds. ).
Heron Press, Sofia, 2004, pp. 93-122.
40. Safarik,I., Safarikova,M.:
Magnetic techniques for the isolation and purification of proteins and
peptides. BioMagn. Res. Technol. 2 (2004) 7 (you can also see the paper here)
41.
Safarikova,M.,
Ptackova,L., Kibrikova,I., Safarik,I.: Biosorption of water-soluble dyes on
magnetically modified Saccharomyces
cerevisiae subsp. uvarum cells.
Chemosphere, in press.
Research
interests
Biomagnetic research and technology
Me and my
colleagues (especially my wife Mirka) from the Laboratory of Biochemistry
and Microbiology are intensively working in the development of magnetic
techniques and their applications in various areas of biosciences,
biotechnology and environmental technology. The complete
list of biomagnetic papers can be found here. Research in the following areas
can be mentioned:
·
Preparation and application of magnetic carriers, adsorbents and
substrates (e.g., chitin [1],
acetylated chitosan [2],
cross-linked chitosan [20],
alginate [37],
dye-stained gelatine [18],
cross-linked erythrocytes [23],
modified Saccharomyces cerevisiae cell
walls [15],
Saccharomyces cerevisiae cells [31,
41],
charcoal [12],
polyphenylene oxide [9],
copper phthalocyanine dye immobilized on magnetic chitosan [4]
or silanized magnetite [5])
·
Isolation of high-molecular-weight biologically active compounds
using magnetic adsorbents (e.g., isolation of hen egg white lysozyme [1,
2],
lysozyme from the gut of the soft tick Ornithodoros
moubata [19],
Solanum tuberosum tuber lectin [20],
α-amylases
[37]
or proteolytic enzymes [23])
·
Isolation of low-molecular-weight organic compounds (e.g.,
tricyclic drugs) and xenobiotics (e.g., dyes) using magnetic adsorbents (e.g.,
adsorption of different organic compounds on magnetic carriers with immobilized
copper phthalocyanine dye [4,
5,
11,
14,
17,
28,
32,
33],
on magnetic charcoal [12,
17],
on magnetic polyphenylene oxide [9]
or on magnetically modified Saccharomyces
cerevisiae cells [31,
41])
·
Isolation of heavy metal ions using magnetic adsorbents [15]
·
Development [17]
and application of Magnetic solid-phase extraction (e.g., for the
preconcentration of malachite green and crystal violet from large volume of
water [28]
or urine [33])
·
Determination of selected xenobiotics using magnetically modified
enzymes [29]
·
Immunomagnetic separation of microbial pathogens [26]
·
Characterization
of magnetically modified microbial cells (e.g., Saccharomyces cerevisiae [36,
38])
·
Study of large-scale separation of magnetic adsorbents [6,
10,
25,
27]
·
Development of magnetic substrates for the determination of enzyme
activity [18]
·
Preparation of review papers and review book chapters summarizing
large parts of biomagnetic research and technology [24]
or their individual areas (e.g., application of magnetic techniques for the
separation of cells [3,
7,
16,
22],
applications of magnetic separation techniques in biochemistry and
biotechnology [8,
13,
21],
isolation of proteins and peptides using magnetic techniques [40]
and relation between magnetic nano- and microparticles and biosciences [30,
34,
39)
·
Setting up a new on-line journal "Biomagnetic Research and Technology",
published at BioMed Central.
Detailed information can be found here and
in the Editorial [35].
·
Attempts
to establish biomagnetic research and technology as a new scientific
discipline.
Applied enzymology and biochemical
analysis
·
Several types of insoluble chromolytic substrates with black
chromogene for the determination of proteinase
(black gelatine), amylase
(black starch), cellulase
(black CM-cellulose) and dextranase
(black dextran) activities were developed. Black gelatin was used for the
determination of
effective
proteolytic activity in biodetergents.
·
Simple
procedures for rapid detection of proteinases (using either gelatine
or fibrin
as substrates), amylases,
dextranases,
proteinase
inhibitors and amylase
inhibitors in liquid samples (e.g., fractions after liquid chromatography)
were developed.
·
Acetylcholinesterases
of various origin were studied (screening
of microbial producers, isolation
from bovine erythrocytes)
·
A
simple procedure for direct
determination of total soil carbohydrate content was also developed.
See the list
of selected papers in this area for further details.
BioMagnetic
Research and Technology: a new online journal
Ivo
Safarik and Mirka Safarikova
Laboratory of Biochemistry and Microbiology, Institute of Landscape Ecology,
Na Sádkách 7,
370 05 Ceske Budejovice, Czech Republic.
Magnetism represents an
interesting phenomenon that has attracted human attention for many years.
Nowadays, magnetism is the basic principle of many devices, procedures and
technologies. However, only relatively recently this phenomenon has also found
really important applications in various areas of biosciences and
biotechnologies.
There are not so many
examples of real magnetic structures connected with living beings.
Nanoparticles composed of magnetic iron oxides have been found in magnetotactic
bacteria, some animals and plants. However, majority of structures found in
living organisms are diamagnetic. Magnetic techniques used in biosciences and
biotechnology are thus predominantly based on magnetic labelling of otherwise
diamagnetic molecules, cell organelles or cells. Magnetic field itself, as well
as magnetic nano- and microstructures can influence specific physiological
functions of living beings under certain conditions.
Biomagnetic techniques have
already been used in different areas of natural sciences such as molecular and
cell biology, medicine, biochemistry, analytical and bioanalytical chemistry,
clinical diagnostics, virology, microbiology, parasitology, immunology,
biotechnology, environmental technology and some others. Nowadays, there is no
publication forum available to cover the entire area of biomagnetic research
and technology. Papers in this area can be found in hundreds of journals,
starting (in alphabetical order) with Abdominal Imaging and ending with Zhurnal
Mikrobiologii, Epidemiologii i Immunobiologii.
In this context, we are
proud to introduce BioMagnetic Research
and Technology http://www.biomagres.com/start.asp,
a new on-line forum for cutting-edge findings in this multidisciplinary area of
research.
As already written on the
WWW pages of BioMagnetic Research and
Technology, this journal is intended as a forum for the disclosure and
discussion of the entire spectrum of biomagnetic research and technology. The
journal will encourage greater interaction between basic and applied research,
as well as cooperation between various scientific disciplines.
The journal will accept
papers on all aspects of biomagnetic research and technology. The following
areas, however, are of special interest:
• biological, medical,
environmental and related systems and processes involving magnetic nano- and
micro structures, and paramagnetic molecules
• the effects of magnetic
fields to biosystems
• construction of magnetic
biocompatible, biodegradable and biomimetic systems
• application of basic
biomagnetic research in biosciences, biotechnology, environmental technology
and related areas
Our new journal will bring
several benefits both to the readers and authors. The papers published in BioMagnetic Research and Technology will
be freely accessible to everybody on the journal WWW page http://www.biomagres.com/start.asp
free of charge. The journal is indexed in PubMed, a well-known on-line medical
database, and the papers will be permanently archived in PubMed Central http://www.pubmedcentral.nih.gov/,
the National Institute of Health's repository for biomedical research articles.
The manuscripts will be submitted and manipulated electronically, saving thus
manipulation delays. There is no limitation as to the article length, number of
colour figures etc., and supplementary or documentary data can be included in
the article. Due to the speed of publication, there will be no loss of priority
of the published results.
As mentioned above, BioMagnetic Research and Technology is
cost-free for the readers, but, unfortunately, it is not cost-free for the
authors. The publishers administration and maintaining the computational
facilities require substantial cost. Therefore there is a flat $500 charge for
each article accepted. However, BioMagnetic
Research and Technology has been granted a moratorium for the first six
months. In addition, Editors are allowed to grant a waiver for a limited number
of authors from disadvantaged countries. We would also like to inform the
future authors that it is possible for their Institutions to become partners of
BioMed Central under very reasonable conditions, leading to the possibility of
all the Institutes researchers to publish free of charge in all the journals
published by BioMed Central:
http://www.biomedcentral.com/info/about/instmembership.
Our aim is to increase the
scientific value of BioMagnetic Research
and Technology. In cooperation with BioMed Central we will look for the
possibility to index our journal also in other databases such as Web of
Science, Chemical Abstracts, Biological Abstracts etc. After an appropriate
period, Institute for Scientific Information will be asked to evaluate our
journal and, if possible, to add it to the list of scientific journals with
Impact Factor. These attempts will, of course, require the active participation
of the authors to submit their high quality papers to this journal, and to cite
the papers published in BioMagnetic
Research and Technology in manuscripts submitted to other journals.
As Editors-in-Chief, and on
behalf of the journal Editorial Board, we welcome all the future authors and
readers to participate in our activity. BioMagnetic
Research and Technology is now the fastest way how to publish results of
your high quality research in various areas of biomagnetic research and
technology, enabling almost unlimited access to your results by your colleagues
and partners.